Barcelona have suffered their first loss under new boss Quique Setién.
The defending La Liga champions were beaten 2-0 by Valencia, following an own goal from Jordi Alba and a strike from Maxi Gomez.
It’s their first defeat at the Mestalla Stadium since 2008.
Barca will lose top spot in the league if Real Madrid takes a point or more at Valladolid on Sunday.
Elsewhere, Sevilla beat Granada 2-0, Espanyol drew 1-1 with Athletic Bilbao and Villareal earned a 2-1 win at Alavés.
In the German Bundesliga, leaders RB Leipzig slumped to a shock 2-0 defeat by mid-table Eintracht Frankfurt, which ended their 13-match unbeaten run in all competitions.
Bayern Munich crushed fifth-placed Schalke 5-0 to close the gap on Leipzig to one point, while Borussia Moenchengladbach came from behind to beat Mainz 3-1.
In Italy’s Serie A, Atalanta forward Josip Ilicic scored a hat-trick, including a stunning free-kick from near the halfway line in his side’s 7-0 win over Torino.
Elsewhere, Genoa fullback Domenico Criscito missed a penalty for the first time in his career as the relegation-threatened team drew 0-0 at Fiorentina, while Bologna beat SPAL 3-1.
Eight teams qualify for Dubai Games finals
Shaidorov shocks rivals to capture gold as 'Quad God' Malinin crumbles
Sabalenka, Swiatek withdraw from Dubai Duty Free Tennis Championships
USA crush Netherlands to get on board at T20 World Cup
