Two-time women's champion Elina Svitolina has crashed out of the Italian Open, while a knee injury has forced Serena Williams to pull out of the tournament.
Fifth seed Svitolina lost 6-4,1-6, 7-5 against Victoria Azarenka in the second round, while Serena announced her withdrawal just before her match against older sister Venus.
Czech fourth seed Karolína Plisková also advanced to the third round along with Australian eighth seed Ashleigh Barty.
In the men's competition, world no. 5 Alexander Zverev suffered a shock 7-5, 7-5 defeat to Italian wildcard Matteo Berrettini, while Belgian David Goffin rallied from a set down to beat Stan Wawrinka 4-6, 6-0, 6-2.
Ninth seed Marin Cilic, seventh seed Juan Martin Del Potro, Australian Nick Kyrgios and Portugal's Joao Sousa are also through to the second round.
Top seed Jannik Sinner ensured Novak Djokovic will be absent from a Wimbledon men's singles final for the first time in eight years after handing the Serbian great a brutal Centre Court battering on Friday.
Fast bowlers Jasprit Bumrah and Jofra Archer took centre stage on the second day of the third test as India and England traded blows at Lord's on Friday and the match remained on a knife-edge.
Twice defending champion Carlos Alcaraz tamed the towering Taylor Fritz to reach his third straight Wimbledon final, rediscovering his A-game when it mattered most to battle past the American in a 6-4 5-7 6-3 7-6(6) victory on Friday.
Ireland's Ben Healy (EF Education-EasyPost) went out alone to win stage six of the Tour de France on Thursday, with Dutchman Mathieu van der Poel (Alpecin-Deceuninck) wrestling back the leader's yellow jersey from reigning champion Tadej Pogacar.